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KMID : 0545120110210050455
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Journal of Microbiology and Biotechnology
2011 Volume.21 No. 5 p.455 ~ p.463
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Genome Organization and Transcription Response to Harvest of Two Metallothionein-Like Genes in Agaricus bisporus Fruiting Bodies
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Daniel Christopher Eastwood
Navdeep K. Bains
Janey Henderson
Kerry S. Burton
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Abstract
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Metallothioneins are a class of small cysteine-rich proteins that have been associated with increased tolerance to metal and oxidative stresses in animals, plants, and fungi. We investigated a metallothionein-like (mt-like) gene shown previously to be upregulated in fruiting bodies of the fungus Agaricus bisporus in response to post-harvest storage. Analysis of an A. bisporus genomic DNA cosmid library identified two similar mt-like genes (met1 and met2) arranged as a bidirectional gene pair transcribed from the same promoter region. The promoter contained regulatory elements including 9 metal responsive elements and a CAAT box region 220 bp downstream of met1 that showed striking similarity to a feature in Coprinopsis cinerea mt-like gene promoters. Transcriptional analysis showed that both met genes are significantly and rapidly (within 3 hours) upregulated during post-harvest storage and expression is significantly greater in stipe and cap tissues compared with the gills. However, a strong directionality of the promoter was demonstrated, as transcript levels of met1 were at least two orders of magnitude greater than those of met2 in all samples tested.
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KEYWORD
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Metallothionein, Agaricus bisporus, bidorectional promoter, Metal Responsive Elements
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